PolyExpress快速兔多克隆抗体服务
蛋白抗原 |
PolyExpress Premium
| PolyExpress Premium Plus |
使用重组蛋白定制抗体**  |
2个亲和纯化多抗
(1-6 mg/个) 及200 ug蛋白抗原 |
1个亲和纯化多抗 (≥2 mg)及1 mg蛋白抗原 |
Guaranteed to perform in antigen ELISA and WB applications |
Guaranteed to perform in antigen ELISA, WB, and IP applications |
|
| |
Peptide antigen |
PolyExpress
Basic |
PolyExpress
Silver |
PolyExpress
Gold
|
针对内源性蛋白设计1条抗原
 |
针对内源性蛋白设计2条抗原
 |
针对内源性蛋白设计3条抗原
 |
针对内源性蛋白产生1个特异性抗体
 |
针对内源性蛋白产生2个特异性抗体
 |
|
承诺 ≥2 mg纯化抗体 |
2 个亲和纯化抗体
(≥2 mg/个) |
3 个亲和纯化抗体
(≥2 mg/个) |
Application Success Rate
 |
|
* Silver及Gold服务套餐仅针对单个的靶蛋白序列，不适用于不同蛋白序列合并的序列。
**抗原蛋白依据方案选择，可能是蛋白全序列或是≥ 10 KD的蛋白片段 。
PolyExpress快速兔多克隆抗体服务详情
| 特色 |
内容 |
Basic
(SC1180) |
Silver
(SC1648) |
Gold
(SC1649) |
Premium (SC1676) |
Premium Plus
(SC1809) |
| 免疫 |
抗原合成 |
1 条肽 |
2 条肽 |
3 条肽 |
1 个蛋白 |
| 免疫兔子 |
2 |
4
(2 只/每条肽) |
6
(2 只/每条肽) |
2 |
| 交付物 |
免疫前血清 |
0.5 ml |
| 抗原 |
2 mg of Each Peptide Antigen |
200 g Protein |
1 mg protien |
| 抗原亲和纯化多抗 |
1
(2-6 mg total) |
2
(4-12 mg total) |
3
(6-18 mg total) |
2
(2-10 mg total) |
1 (2 mg) |
| 承诺 |
应用 |
ELISA 效价 1:64,000 |
免疫原WB (Premium套餐) 或 WB & IP (Premium Plus套餐) 阳性，并且ELISA效价 1:128,000 |
| 交付抗体 |
1 个纯化多抗 (≥2 mg) |
2个纯化多抗
(≥2 mg/个) |
3 个纯化多抗
(≥2 mg/个) |
2 个纯化多抗(1-6 mg/个) |
1 个纯化多抗
(≥2 mg) |
| 交付时间 |
45 天 |
10-11 周 |
 | Our OptimumAntigen Design Tool combines the industry's most advanced algorithms with GenScript's professional expertise resulting in: - Strong Antigenicity
- Avoidance of non-surface epitopes
- Specify desirable cross-reactivity
- Elimination of re-synthesis delays with a built-in peptide tutorial for synthesis and solubility
- Choose the best antigen conjugation and presentation options for your desired assay(s)
- A guaranteed immune response
|
 | Our PolyExpress Gold package increases success rate by using more than one antigen peptide for each protein  |
 | A proprietary immunization adjuvant elicits a stronger and faster cellular immune response with longer immune sustainability, making the production of high-titer, high affinity custom antibodies possible.
| *High Immunoaffinity | | *Increase response | | *Diminished toxicity | |
| | |
 |  |
Customer case study
Western blot analyses were performed to measure the effect of the Osi21 functional loss on the degradation of endocytosed rhodopsin.
The polyclonal rabbit antiarrestin2 antibody is diluted 1:30,000. (GenScript, NY)
J Lee, M Song, S Hong. Negative Regulation of the Novel norpAP24 Suppressor, diehard4, in the Endo-lysosomal Trafficking Underlies Photoreceptor Cell Degeneration. PLoS Genet. June 2013, 9, e1003559
Experimental Data
Antibodies produced using PolyExpress have ELISA titers as high as polyclonal antibodies produced using a traditional protocol.
ELISA analysis of purified antibody generated by either PolyExpress protocol or traditional protocol.
ELISA analysis of purified antibody generated by PolyExpress Antibody Service.
PolyExpress generated an antibody with ELISA titer> 1: 256,000 which is higher than guaranteed.
